av E Bengtsson · 2009 — Methods. 22. DNA extraction. 22. SSR markers. 22. PCR. 23 and Cross-Species Amplification in. P. ssp.. Crop Science 42, 2128-2136.

HLA typing by sequence-specific primers (PCR-SSP) Amplification with sequence-specific primers yields only a product if the target sequences are present in the DNA sample (compare lane 7 and 8 with the figure) In total 16 primers are used for the analysis of HLA-DR4 allele.

Our main aim was to standardize a simple inexpensive in-house PCR-SSP technique for HLA-B* 27 typing. Materials and Methods. We have developed a method that allows the amplification of double-stranded DNA even when the sequence information is available at one end only ( 5 ). This method, the single specific primer-PCR (SSP-PCR), permits amplification of genes for which only a partial sequence information is available, and allows unidirectional genome walking from known into unknown regions of the chromosome.

In-house PCR-SSP technique is very simple and inexpensive technique to detect B* 27 allele, which was strongly associated with SpA patients from Western India. Background. Microlymphocytotoxicity (MLCT) and flowcytometry (FC) are the conventional serological methods to detect HLA-B* 27. Since PCR-SSP is DNA-consuming, this procedure requires at least 500 ng genomic DNA; this, however, may be critical with regard to a retrospective analysis, or when valuable samples need to be analyzed.

Single Specific Primer-PCR (SSP-PCR): allows the amplification of double-stranded DNA even when the sequence information is available at one end only.

The Polymerase Chain Reaction (PCR). © 2006 Sumanas, Inc. KEYWORDS: Polymerase chain reaction, DNA amplification, Taq polymerase, genomics

Designed to address the diverse needs of HLA labs, our molecular product family includes Next Generation Sequencing (NGS), sequence-based typing (SBT), Real-Time PCR (qPCR), sequence-specific primers (SSP), and reverse sequence-specific oligonucleotides (rSSO). Several PCR-based techniques have been devised to predict D phenotype.

Commonly used DNA based HLA typing methods include PCR based sequence specific primers (PCR-SSP), and PCR based restriction fragment length

Materials and Methods. We have developed a method that allows the amplification of double-stranded DNA even when the sequence information is available at one end only ( 5 ). This method, the single specific primer-PCR (SSP-PCR), permits amplification of genes for which only a partial sequence information is available, and allows unidirectional genome walking from known into unknown regions of the chromosome. The Join- point and the adjacent DNA can be identified by SSP-PCR, as long as sequence information is avail- able on one side or the other of the join-point.

Its origins are probably legion, i.e. many people probably thought of it at the same time. Clinically, 54% of patients had polyarticular arthritis with SI joints involvement (68%) and restricted spine flexion (60%). Conclusion. In-house PCR-SSP technique is very simple and inexpensive technique to detect B* 27 allele, which was strongly associated with SpA patients from Western India.
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PCR-SSP abbreviation stands for Polymerase … The polymerase chain reaction with sequence-specific primer (PCR-SSP) is currently used for HLA-B27 testing. The purpose of this study was to screen for HLA-B27 in DNA samples from 350 Thai blood donors using PCR-SSP technique and compare the results with those obtained from hospital patients .

Key words: Brucella spp. , milk, cheese, Hemi Nested PCR, Nested PCR The advantages: Readily analysed by PCR and easily detected on PAGE SSLPs with large size differences - detected on agarose gels SSR markers can be multiplexed (by pooling independent PCR products or by true multiplex-PCR) genotyping throughput is high and can be automated start-up costs are low for manual assay methods (once the markers have been developed) SSR assays require only very small 46 Nathalang O, Tantimavanich S, Nillakupt K, Arnutti P, Jaruchaimontree C. HLA-B27 testing in Thai patients using the PCR-SSP technique. Tissue Antigens.
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We have developed a polymerase chain reaction-based technique, using sequence- specific primers (PCR-SSP) for HLA typing, starting with the HLA class II

For e.g. the primers CONCLUSION: PCR‐SSP is a helpful supplementary technique for resolving most of the common problems caused by discrepant or doubtful serologic results, and it is an easy‐to‐handle robust method. Questionable cases in donor, recipient, and patient typing can be examined with acceptable cost.